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Performance requirements for ideal HTS assays
| Parameter | Performance standard |
|---|---|
| S : B | > 3 |
| separation band or assay window | 1 SD between the mean maximum value - 3 SD and the minimum value + 3 SD |
| Z-factors | greater than 0.5 |
| intra-plate variability | < 20% COV |
| inter-plate variability | each plate within 20% of the mean of the 2 plates |
| day-to-day variability | < 20% COV between plate pairs |
SD, standard deviation
COV, coefficient of variance
