Figure 3. aPKC’s effects on polarity cues.

(A) Arm colocalizes with Baz in puncta in gastrulating apkc^m/z mutants (arrows). (B) Arm in WT spot junctions. (C) Lack of PAR-6 and (G) Crb enrichment at Baz puncta in gastrulating apkc^m/z mutants. Both have reduced cortical enrichment at both gastrulation (C, G) and after germband extension (E, I) versus WT (D, F, H, J). (K) Dlg overlaps with DE-Cad puncta in gastrulating apkc^m/z mutants and localizes to apical surface membrane (arrow), as in WT (L, arrow; inset shows a side view at this stage). (M) Dlg continues to localize to apical surface membrane after apkc^m/z mutant germband extension (arrow; inset shows a side view at this stage), in contrast to WT where Dlg localizes below DE-Cad (N, a slightly oblique section in which the top of the panel is above the embryo surface; inset shows a side view at this stage). Bar, 5μm.