Figure 6. The roles of Dynein and MTs in AJ clustering in apkc^m/z mutants.

(A) During WT gastrulation, Dynein heavy chain has an even cytoplasmic distribution with some enrichment at the cortex in proximity to AJs and Baz (Dynein intermediate chain has a similar distribution (data not shown)). (B, C) During apkc^m/z mutant gastrulation, both Dynein heavy chain (B) and Dynein intermediate chain (C) have even cytoplasmic and cortical distributions. (D) At full WT germband extension, the Dynein distribution remains even. (E, F) At full apkc^m/z mutant germband extension, Dynein heavy chain (E) and Dynein intermediate chain (F) show specific enrichment at fragmented AJ/Baz complexes (arrows). (G-I) apkc^m/z mutants, DE-Cad (blue), Baz (red), MTs (green). (G) DE-Cad/Baz puncta disperse as MTs (blue) are lost from the cortex when spindles form in the first mitotic domains (compare mitotic cells, left (dispersing complexes bracketed), with cells yet to divide, right (arrows, intact complexes)). (H) Colchicine-treatment (30 min) of gastrulating apkc^m/z mutants induces MT (blue) loss in most cells and a corresponding dispersion of DE-Cad/Baz puncta (bracketed). Arrows, complexes retained in cells with residual MTs. (I) Ethanol carrier alone. Bar, 5 μm.