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. Author manuscript; available in PMC: 2008 May 15.
Published in final edited form as: J Neurosci Methods. 2007 Jan 16;162(1-2):171–179. doi: 10.1016/j.jneumeth.2007.01.005

Figure 4.

Figure 4

Flies recover from a second dose of ethanol more quickly than from a first dose. These composite images come from subtracting the first image of the sequence from all the subsequent images (using compare2first.pl). Images are shown at five minute intervals. The flies in (a) are recovering from their first ethanol sedation, and the flies in (B) were sedated four hours earlier, making this their second recovery.