Figure 1.

WAP/bTAP construct and transcript in lactating mammary gland. (A) Schematic representation of the WAP/bTAP transgene. Shaded box, WAP promoter; solid box, WAP exons, hatched box, bTAP exons; line, introns and 3′ untranslated sequence. (B) Total RNA (1 μg, lanes A, C, E, G, I, and K; 5 μg, lanes B, D, F, H, J, and L) was loaded for each sample. Samples from the 3880 line (lanes A–D) founded by a female and from the 3863 line (lanes E–L) founded by a male are included. F₁ females judged to be transgenic (+) by PCR were assayed and contained the bTAP transcript (lanes C–F, K, and L). Nontransgenic siblings [(−) by PCR] from the F₁ generation of each line were included as negative controls (lanes A, B, and G–J). The blot was probed with a random primed bTAP genomic probe. A 5-hr exposure is shown. The bTAP transcript is indicated by arrow on right.