Table 1.

Frequency of P1 transduction of plsB::kan, plsX::kan, and ygiH::kan mutations into W3110 cells harboring various plasmids

Recipient strain									Relative transduction frequency*
	Chromosomal gene		Harboring plasmids						P1 donor strain
Strain	plsX	ygiH	pSTV29	pSTV29 plsB	pSTV28 EcplsX	pSTV29 ygiH	pSTV28 BsplsX	pSTV29 yneS	ΔplsB::kan	ΔplsX::kan	ΔygiH::kan
W3110	+	+	-	-	-	-	-	-	0	100	100
MEC104	+	+	+	-	-	-	-	-	0	50	64.3
MEC105	+	+	-	+	-	-	-	-	100	65.5	58.9
MEC106	+	+	-	-	+	-	-	-	0	80	50.9
MEC107	+	+	-	-	-	+	-	-	0	100	78
MEC108	+	+	-	-	-	-	+	-	0	63.6	100
MEC109	+	+	-	-	-	-	-	+	0	81.8	70.6

*Transduction frequencies are expressed as percentages of the highest number of Km^r colonies recorded in each experiment, and are depicted as blocks.