Table 2.
Frequency of P1 transduction of plsB::kan, plsX::kan, and ygiH::kan mutations into strains MEC102 and MEC103 harboring various plasmids
| Recipient strain | Relative transduction frequency* | ||||||||||
| Chromosomal gene | Harboring plasmids | P1 donor strain | |||||||||
| Strain | plsX | ygiH | pSTV29 | pSTV29 plsB | pSTV28 EcplsX | pSTV29 ygiH | pSTV28 BsplsX | pSTV29 yneS | ΔplsB::kan | ΔplsX::kan | ΔygiH::kan |
| MEC102 | - | + | - | - | - | - | - | - | 0 | 100 | 0 |
| MEC103 | + | - | - | - | - | - | - | - | 0 | 0 | 100 |
| MEC110 | - | + | + | - | - | - | - | - | 0 | 55.9 | 0 |
| MEC111 | - | + | - | + | - | - | - | - | 100 | 53.5 | 59.8 |
| MEC112 | - | + | - | - | + | - | - | - | 0 | 56.3 | 75.3 |
| MEC113 | - | + | - | - | - | + | - | - | 0 | 83.1 | 100 |
| MEC114 | - | + | - | - | - | - | + | - | 0 | 45.1 | 84.2 |
| MEC115 | - | + | - | - | - | - | - | + | 0 | 100 | 92.7 |
| MEC116 | + | - | + | - | - | - | - | - | 0 | 0 | 69.8 |
| MEC117 | + | - | - | + | - | - | - | - | 100 | 63.7 | 85.9 |
| MEC118 | + | - | - | - | + | - | - | - | 0 | 99.5 | 100 |
| MEC119 | + | - | - | - | - | + | - | - | 0 | 85.1 | 73.8 |
| MEC120 | + | - | - | - | - | - | + | - | 0 | 36.3 | 60.5 |
| MEC121 | + | - | - | - | - | - | - | + | 0 | 100 | 96 |
*Transduction frequencies are expressed as percentages of the highest number of Kmr colonies recorded in each experiment, and are depicted as blocks.