Table 1.

Clinical disease following inoculation of CWD brain into deer PrP tg mice^a

Inoculate	Route of inoculation	Recipient mice	Infected mice totalb	Mean ± SD when mice were sacrificed
CWD brain	i.c.	PrPko	0/5	≥ 700
		+/− line 33	13/13	312 ± 32
		+/− line 39	15/15	275 ± 46
		+/+ line 39	5/5	210 ± 15
		C57Bl/6	0/6	≥ 500
PBS	i.c.	PrPko	0/5	≥ 600
		+/− line 33	0/4	≥ 600
		+/− line 39	0/4	≥ 600
		+/+ line 39	0/4	≥ 600
		C57Bl/6	0/4	≥ 600
CWD brain	oral	PrPko	0/5	≥ 600
		+/+ line 33	3/3	381 ± 55
		+/+ line 39	9/9	370 ± 26
		C57Bl/6	0/4	≥ 600
PBS	oral	PrPko	0/2	≥ 640
		+/+ line 33	0/2	≥ 640
		+/+ line 39	0/2	≥ 640
		C57Bl/6	0/2	≥ 640

^aPrPko, heterozygous (+/−) or homozygous (+/+) deer PrP tg, or C57Bl/6 mice were inoculated i.c. or orally with homogenate made from pooled brains obtained from mule deer dying from CWD. Recipient mice were followed for signs of disease that included ataxia, tremors, weakness, lethargy, and kyphosis.

^bNumbers of mice having clinical signs and on sacrifice showing PrPres by both Western blot and immunohistochemistry over the total inoculated. Those not showing clinical signs (PrPko and C57Bl/6 mice) were sacrificed at times indicated. Mice displaying clinical signs were sacrificed either early in disease or when moribund.