Percentage FUS1-lacZ induction of receptors containing asparagine and glutamine substituted for aspartic and glutamic acid residues in response to 2 μM α-factor. The results are shown as mean ± SEM of at least three independent experiments. The strains expressing the empty vector and the wild type receptor are same as used in Figure 2. The strains expressing D3N, D14N, D39N, E40Q, D124N, D195N, D201N, and D275N mutant receptors are A2798, A2799, A2800, A2801, A2802, A2803, A2804, and A2805 respectively.