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. Author manuscript; available in PMC: 2007 Aug 20.
Published in final edited form as: Biochim Biophys Acta. 2007 Feb 17;1773(6):707–717. doi: 10.1016/j.bbamcr.2007.02.002

Figure 6.

Figure 6

pH-dependence of binding of [K7(NBD), Nle12] α-factor to normal and D275N mutant receptors. Data points represent the mean ± SEM for three independent isolates of each strain. Binding of [K7(NBD), Nle12] α-factor to strains A448 (STE2+) and A2805 (D275N) was assayed at pH 4.5 (panel A), pH 5.8 (panel B), and pH 7.2 (panel C). Solid lines indicate the results of least squares fitting to the data for strain A448 as described in Materials and Methods. Dashed lines represent the fit to data for strain A2805. The data for strain A448 at all pHs and for strain A2805 at pH 4.5 was fit as single site binding with a nonspecific component as described in Materials and Methods. The data for strain A2805 at pH 5.8 and pH 7.2 was fit to a linear regression passing through the origin.