Table 3.
Relative activity in a growth arrest assay of STE2 alleles containing substitutions at extracellular charged groups
| Yeast strain | STE2 allele | Relative activity* |
|---|---|---|
| A448 | STE2+ | 100 |
| A2360 | D3A | 56 ± 10 |
| A2361 | D14A | 49 ± 6** |
| A2362 | D39A | 51 ± 7** |
| A2363 | E40A | 55 ± 7** |
| A2364 | D124A | 50 ± 6** |
| A2365 | D195A | 48 ± 6** |
| A2366 | D201A | 46 ± 5** |
| A2367 | D275A | N.D. † |
| A2798 | D3N | 79 ± 12 |
| A2799 | D14N | 70 ± 10 |
| A2800 | D39N | 77 ± 11 |
| A2801 | E40Q | 76 ± 11 |
| A2802 | D124N | 87 ± 13 |
| A2803 | D195N | 82 ± 11** |
| A2804 | D201N | 73 ± 13 |
| A2805 | D275N | N.D. ‡ |
| A3081 | K100A | 89 ± 15 |
| A3082 | R122A | 106 ± 18 |
| A3083 | H126A | 144 ± 8 |
| A3084 | K187A | 136 ± 24 |
| A3085 | K202A | 95 ± 16 |
| A3086 | K269A | 101 ± 26 |
Relative activity is calculated as: (α-factor required to produce a 15 mm halo in the wild type Ste2p/α-factor required to produce a 15 mm halo in the mutant alleles) × 100.The values are expressed as mean ± SEM of at least three independent experiments.
The change activity for these alleles (compared with STE2+) was statistically significant at the level of p<0.05.
N.D., Not Determined; Halos for this strain had indistinct edges and had smaller diameters than halos for strains expressing wild-type receptors.
N.D., Not Determined; Halos for this strain had indistinct edges but were approximately the same size as the halos of strains expressing wild-type receptors.