Figure 3. Effect on transfection with HK polyplex formed by sequential addition of water followed by increasing NaCl concentrations.

HK polyplex formed first in water for 30 min followed by addition of NaCl solutions varying from 100 for 600 mM for 15 min. Twenty‐four hours after complexes were added to medium, luciferase activity was measured. The complexes in which NaCl concentrations of 200 to 600 mM were added (final concentration of NaCl in which polyplex was formed was one‐half these) had the highest transfection activity. *, P<0.01, water/NaCl ≥ 200 mM vs water; multiple comparison test compared to Control (water) Group (Bonferonni t‐test).