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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Methods. 2007 Jul;42(3):278–288. doi: 10.1016/j.ymeth.2007.02.005

Table 1.

Kinetic constants for inhibitor-sensitized PTP1B mutants, assayed with pNPP as substrate at pH 5.2

PTP kcat (s−1) KM (mM) kcat/KM (s−1 mM−1)
wild-type PTP1B 12 ± 1.8 0.32 ± 0.067 40
V49A PTP1B 11 ± 3.7 2.2 ± 0.057 5.5
I219A PTP1B 24 ± 1.3 1.4 ± 0.35 17