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. Author manuscript; available in PMC: 2007 Aug 21.
Published in final edited form as: Biochem Pharmacol. 2006 Dec 28;73(9):1474–1481. doi: 10.1016/j.bcp.2006.12.026

Figure 2. GSSG-mediated time- and concentration-dependent inactivation of 17β-estradiol sulfation catalyzed by purified hSULT1E1 and by SULTs in human liver cytosol.

Figure 2

Human liver cytosol (final concentration, 1.0 mg/ml) or purified hSULT1E1 (final concentration, 0.1 mg/ml) were incubated in Tris buffer (pH 6.2) at room temperature with various GSSG concentrations and durations, as indicated. [3H]E2 sulfation (hSULT1E1) activity was determined using the radioactive assay method [21, 22]. The apparent first-order rate constant (Kapp) in Figure 2C and 2D was calculated using data shown in Figure 2A and 2B, respectively.