Skip to main content
. 2007 Aug 23;117(9):2602–2610. doi: 10.1172/JCI30842

Figure 5. In vitro effects of Pax5 and CD22.

Figure 5

(A) Cell accumulation in untreated and 4OHT-treated cultures of Pax5ER and GFP-only Myc5-M5 cells. Cells were counted in triplicate; error bars denote SD. Insets represent 3H-thymidine incorporation assay performed on the same cultures. (B) Cell accumulation in untreated and 4OHT-treated cultures of CD22- and vector-transduced Myc5-Myc5–Pax5ER cells. (C) Pax5 knockdown in human SUDHL-4 and SUDHL-6 DLBCL lines using lentivirally encoded shRNAs. Five different hairpins were first tested on SUDHL-4 cells. The most effective shRNA, 6061, was also introduced into SUDHL-6 cells. Pax5 and actin levels were determined using immunoblotting. (D) Cell accumulation assay following Pax5 knockdown. Cells transduced by shRNA 6061 and control virus were seeded at equal densities and counted 48 hours later. P values were determined using unpaired Student’s t test.