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. 2007 Aug 23;117(9):2583–2591. doi: 10.1172/JCI31892

Figure 4. Changes in circulating NOx metabolites.

Figure 4

Plasma (A, C, E, and G) and rbc (B, D, F, H, and I) of nitrate (A and B), nitrite (C and D), SNO (E and F), XNO (G and H), and HbNO (I) are shown as a function of blood draw in placebo (filled squares) and iNO (filled circles) groups. Plasma NOx levels were normalized to protein. Absolute concentration ranges (minimum to maximal) were: nitrate (μM), placebo 47.9 ± 15.7 to 54.7 ± 19.2, iNO 84.6 ± 32.5 to 140.3 ± 24.5; nitrite (nM), placebo 202 ± 38.4 to 381.3 ± 49.3, iNO 462.2 ± 181.13 to 923.1 ± 240; SNO (nM), placebo –0.16 ± 0.17 to 1.3 ± 0.66, iNO 0.05 ± 0.2 to 2.3 ± 0.66; XNO (nM), placebo, 0.29 ± 0.2 to 3.5 ± 0.58, iNO 1.04 ± 0.35 to 4.7 ± 1.9. rbc NOx levels were normalized to heme. Absolute concentrations (in packed rbc) were: nitrate (μM), placebo 31.7 ± 12.5 to 41.5 ± 19.1, iNO 80.3 ± 48.3 to 196.2 ± 44.3; nitrite (nM), placebo 646.1 ± 80.8 to 1,219.5 ± 131.7, iNO 1,241.4 ± 447.7 to 2,533.3 ± 681.1; SNO (nM), placebo 4.9 ± 2.5 to 6.2 ± 2.2, iNO 29.1 ± 16.3 to 40.7 ± 18.2; XNO (nM), placebo 0.19 ± 0.15 to 1.8 ± 1.2, iNO 7.3 ± 4.9 to 13.4 ± 9.3; HbNO (nM), placebo 93.4 ± 4.4 to 242.8 ± 32.9, iNO- 222.9 ± 53.2 to 764.6 ± 108.1. Differences were assessed by 2-way repeated measures ANOVA and Bonferroni post-test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 for placebo versus iNO at corresponding blood draw. (J) Plasma and rbc A-V gradients at BD3 for indicated NO intermediates. Concentration units for each NOx are as indicated in respective panels showing changes in individual NOx as a function of BD (A–I). *P ≤ 0.001 for placebo versus iNO for plasma nitrite. All values are mean ± SEM; n = 10, and n = 6–7 for HbNO.