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. 2007 Jul 10;35(14):4858–4868. doi: 10.1093/nar/gkm492

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — There is a functional SRF binding site in the LXRB gene promoter. (A) An oligo covering the wild type LXRB gene promoter Elk1 binding site (WT Elk1-oligo) and a mutated Elk1 site (Mut Elk1-oligo) was incubated with IVT Elk1 (lanes 3 and 7) or IVT SRF (lanes 4 and 8) or the empty control plasmid (lanes 2 and 6). (B) The WT Elk1-oligo was incubated with WCE from the INS1 cell line (lane 1) IVT Elk1 (lane 2), IVT SRF (lane 3) or both (lane 4). (C) Empty vector or a vector containing Elk1 or SRF were in vitro transcribed/translated and separated on a 8% gel to monitor the levels of expression.