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. 2007 Jul 10;35(14):4905–4913. doi: 10.1093/nar/gkm473

Figure 5.

Figure 5.

Transcription of virF mRNA. (A) In vitro transcription of virF mRNA visualized with ethidium bromide on a 1% TAE agarose gel. The single-stranded mRNA ran at ∼500 bp in comparison to the 100-bp DNA ladder (lane 1). Lane 2: virF mRNA from a previous transcription reaction. Lanes 4 and 5: virF mRNA from replicate transcription reactions. (B) RT–PCR samples visualized with ethidium bromide on a 1% TAE agarose gel. RT–PCR was conducted using extracted mRNA from replicate samples (lanes 2 and 3), electrophoresed in comparison to a 100 bp DNA ladder (lane 1). Each RNA sample produced the corresponding 800 bp DNA species indicative of the virf ORF.