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RNA kinetic parameters with E. coli htTGT(wt) and [3H] preQ1 at pH 7.3
| KM (μM) | kcat (10−4 s−1) | kcat/KM (102 M−1 s−1) | |
|---|---|---|---|
| ECY | 0.16 (0.03)a | 40 (2) | 250 (40) |
| dG34ECYMH | 0.26 (0.07) | 70 (5) | 270 (60) |
| virF mRNA | 1.8 (0.4) | 1.1 (0.1) | 0.60 (0.1) |
| virF MH | 0.87 (0.3) | 3.3 (0.3) | 3.8 (1) |
aStandard errors are in parentheses.
