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. 2007 Jul 10;35(14):4869–4881. doi: 10.1093/nar/gkm517

Figure 4.

Figure 4.

Effects of alanine-scanning mutations within the first finger on the activity of TFIIIA. (A) Abilities of versions of TFIIIA with alanine-scanning mutations to bind to the 5 S RNA gene and to recruit TFIIIC to the TFIIIA–DNA complex as assessed by EMSA. See Figure 1E for details. The portion of the gel containing the TFIIIC–TFIIIA–DNA complex is shown at a higher exposure above the full-sized gel. (B) Abilities of mutant versions of TFIIIA to support in vitro transcription of the 5 S RNA gene. See Figure 1D for details. (C) Abilities of mutant versions of TFIIIA to support cell viability. See Figure 1G for details. (D) Assessment by western blot analysis of in vivo expression of mutant versions of TFIIIA from the ΔpG3 series of plasmids. See Figure 1G for details.