Figure 3.

Transfection of pGL4-based bicistronic vectors into MCF7 cells. (A) Diagram of bicistronic constructs. (B) The left panel shows activity of the upstream cistron (Firefly luciferase) and the right panel shows activity of the downstream cistron (Renilla luciferase). Bars labeled 472 indicate constructs with the p27 5′-UTR in the forward orientation and bars labeled 472R indicate constructs with the p27 5′-UTR in the reverse orientation. For bars labeled −Promoter cells were transfected with constructs lacking a promoter upstream of the first cistron and for bars labeled +Promoter cells were transfected with constructs carrying the SV40 promoter. The number above each bar indicates the mean of three separate transfections. The error bars represent SD.