Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Jun 22;35(14):4608–4618. doi: 10.1093/nar/gkm481

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Characterization of BsrDI A/B subunits. (A) Purified recombinant BsrDI A and B subunits. The calculated molecular weight of BsrDI A and B subunits are 25 and 56 kDa, respectively. Lane 1, protein size marker; lane 2, BsrDI B subunit (Nb.BsrDI); lane 3, BsrDI A subunit. (B) Reconstituted BsrDI double-strand cleavage activity by mixing purified BsrDI A and B subunits. Lane 1, 1-kb DNA marker; lane 2, pUC19; lane 3, 8 nM pUC19 treated with A subunit; lanes 4–8, 8 nM pUC19 digested with A and B subunits (linear pUC19 = 2.7 kb); sc, supercoiled DNA. (C) Nb.BsrDI DNA nicking activity on 8-nM pUC19. Lane 1, 1-kb DNA marker; lane 2, pUC19; lanes 3–9, pUC19 digested with BsrDI B subunit, enzyme concentration as indicated on the top of each lane.