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. 2007 Jun 22;35(14):4608–4618. doi: 10.1093/nar/gkm481

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — (A) DNA nicking or ds cleavage activity of BsrDI A/B mutants. B cat* = catalytic-deficient B subunit. Plasmid pUC19 (2 BsrDI sites) was used as the substrate for nicking or restriction assay. NC, nicked-circular DNA; SC, supercoiled DNA. (B) Run-off sequencing of gel-purified, nicked-circular DNA. The expected nicking specificity of Nt.BsrDI is GCAATGNN↓ (BsrDI site and the adjacent sequence are shown at the bottom of the figure). The extra A peak at the end of run-off sequence was added by the template independent terminal transferase activity of the Taq DNA pol.