Figure 2.

Synthesis of NS1 and NEP(NS2) proteins from the recombinant replicons. Cultures of HEK293T cells were transfected with the appropriate plasmids to establish the indicated wt or mutant NS replicons or mock transfected (Mock). At 36 h post-transfection, the cultures were pulse labelled with ³⁵S methionine-cysteine and whole cell extracts were analysed by polyacrylamide gel electrophoresis and autoradiography. Cells containing the NS1 81 or the 11C replicons were incubated at either permissive (32) or restrictive (39) temperature. The position of the relevant proteins is indicated to the right. The mobility of molecular weight markers in shown to the left.