Figure 4.

Intracellular localization of NS1 protein expressed from recombinant NS replicons. Cultures of HEK293T cells were transfected with the appropriate plasmids to establish the indicated wt or mutant NS replicons or mock transfected (Mock). At 36 h post-transfection, the cultures were fixed and processed for immunofluorescence using anti-NS1 serum (NS1). The cultures were counterstained with Dapi to reveal the nuclei (Dapi). Cells containing the NS1 81 or the 11C replicons were incubated at either permissive (32) or restrictive (39) temperature.