Figure 5.

Stability of NS-encoded viral mRNAs. (A) Cultures of HEK293T cells were transfected with the appropriate plasmids to establish the wt or ΔNS1 NS replicons. At 36 h post-transfection, actinomycin D was added to the cultures and total RNA was extracted at the times thereafter indicated in the Figure. The poly A-containing RNA was selected by oligo dT-cellulose chromatography and the amounts of NS1 and NEP mRNA were determined by RNAse-protection assay. The results from two independent experiments are shown and the half-lives obtained from each experiment are presented in the part B of the Figure.