Table 1.
Comparison of mRNA expression of mechanosensitive proteins from the EPI and ENDO region of rat left ventricle
| Assay ID reference | Common name | EPI | ENDO | Paired t test |
|---|---|---|---|---|
| Mean ± SEM | Mean ± SEM | |||
| Slow response and/or hypertrophic | ||||
| Agtr1a-Rn00578456_m1 | Angiotensin II receptor 1A | 1.64 ± 0.16 | 1.30 ± 0.22 | NS |
| Edn1-Rn00561129_m1 | Endothelin-1 | 1.17 ± 0.14 | 1.58 ± 0.24 | NS |
| Ednra-Rn00561137_m1 | Endothelin-1 receptor type A | 1.19 ± 0.14 | 1.38 ± 0.12 | NS |
| Ednrb-Rn00569139_m1 | Endothelin-1 receptor type B | 1.27 ± 0.17 | 1.93 ± 0.22 | 0.001 ENDO > EPI |
| Nppb-Rn00580641_m1 | Brain natriuretic peptide | 1.42 ± 0.15 | 2.77 ± 0.42 | 0.002 ENDO > EPI |
| Slc9a1-Rn00561924_m1 | Sodium–hydrogen exchanger | 1.33 ± 0.09 | 1.51 ± 0.17 | NS |
| Myofilaments | ||||
| Myh6-Rn00568304_m1 | α-myosin heavy chain | 1.56 ± 0.12 | 1.37 ± 0.17 | NS |
| Myh7-Rn00568328_m1 | β-myosin heavy chain | 1.20 ± 0.23 | 2.19 ± 0.36 | 0.006 ENDO > EPI |
| Mechanosensitive channels | ||||
| Kcnk2-Rn00597042_m1 | Kcnk2 (TREK-1) | 0.84 ± 0.04 | 1.04 ± 0.04 | 0.001 ENDO > EPI |
| Trpc1-Rn00585625_m1 | Transient receptor potential canonical-1 | 0.72 ± 0.04 | 0.72 ± 0.05 | NS |
| Mechanotransducers (caveolae) | ||||
| Cav-Rn00755834_m1 | Caveolin-1 | 0.90 ± 0.03 | 0.96 ± 0.03 | 0.015 ENDO > EPI |
| Cav3-Rn0055343_m1 | Caveolin-3 | 0.81 ± 0.03 | 0.85 ± 0.05 | NS |
Data shown are mean ± SEM, expression is relative to GAPDH. The probability of paired t tests is shown in the table. P > 0.05 (n = 10 pairs).
NS Not significantly different