. 2007 Mar 1;116(4):385–402. doi: 10.1007/s00412-007-0103-y

Table 1.

Pairwise mapping of CKC components

Red protein	Green protein	d_RRinter^a (nm)	d_GGinter^a (nm)	d_RGintra^a (nm)
Cenp-A/Cid	Cenp-A/Cid	445	455	5
	Cenp-C(C)^b	460	490	15
	Cenp-C(N)^b	463	547	42
	Mis12	472	574	51
	Nuf2	451	589	69
Spc25	Cenp-A/Cid	573	475	−49
	Cenp-C(C)^b	556	511	−23
	Cenp-C(N)^b	539	512	−14
	Mis12	503	516	7
	Nuf2	547	576	15
	Spc25	531	531	0
(N)^c-Cenp-C-(C)^c		575	559	−8
(C)^d-Cenp-C-(N)^d		461	491	15

^aNative chromosomes (n = 100) from embryos coexpressing a red and a green fluorescent CKC component were analyzed. Average values for the distances separating the red fluorescent signal maxima of sister kinetochores (d_RRinter) and the distances separating the green fluorescent signal maxima of sister kinetochores (d_GGinter) were used for the calculation of the distance separating the two components within a sister kinetochore (d_RGintra; see also Fig. 7d and experimental procedures)

^bCenp-C was expressed as a fusion with EGFP either at the N terminus [Cenp-C(N)] or at the C terminus [Cenp-C(C)]

^cCenp-C was expressed with mRFP and EGFP at the N and C termini, respectively

^dCenp-C was expressed with EGFP and mRFP at the N and C termini, respectively