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RNase protection analysis of the c-fos gene expression in the striatum of normal awake rats. Different amounts of total RNA from rat striatum were analyzed by using 32P-labeled antisense rat c-fos riboprobe (lanes 1–4: 1, 2, 5, and 10 μg, respectively). The 557-nt protected fragment is clearly visible with 2 μg of total RNA after autoradiography at −80°C for 72 h. The yeast RNA lane (Y) serves as a negative control.
