Figure 6.

Effects of intrastriatally injected c-fos antisense (▪, n = 10), sense (▾, n = 5), and random (▴, n = 2) oligonucleotides (2 nmol) on GABA release in the SN of awake, freely moving rats. The sham group (○, n = 5) was infused with Ringer solution alone. The vertical arrow indicates the onset of the intrastriatal injection with the oligonucleotide. The results are expressed as a percentage of three basal values measured prior to the oligonucleotide injection. Basal nigral GABA levels measured in 30 min perfusate fractions were 7.9 ± 0.2 nM. Student’s paired t test, comparing the overall mean of pre- and the postinjection periods, showed that only the intrastriatally injected c-fos antisense oligonucleotide induced a significant decrease in nigral basal GABA levels (P < 0.001). Two-way ANOVA followed by Newman–Keuls test for multiple comparisons showed a significant difference between treatments (P < 0.001), with the antisense group being significantly different from all the other groups.