Table 1.

RNase protection analysis of striatal c-fos, jun B, an dNGFI-A mRNA levels after treatment with c-fos antisense, sense, and scrambled oligonucleotides

Treatment	n	c-fos	junB	NGFI-A
Ringer	3	100  ±  9.4	100  ±  10.5	100  ±  17.9
Antisense	6	202  ±  16.8*	247  ±  41.2*	214  ±  30.8*
Sense	4	139  ±  6.5	152  ±  12.9	124  ±  13.0
Scrambled	4	145  ±  19.9	152  ±  14.3	123  ±  7.4

Oligonucleotides (2 nmol) were intrastriatally injected into conscious rats and striatal tissue was prepared 90 min later. Values represent the mean (±SEM) ratio of the signal obtained with a ³²P-labeled c-fos-, junB-, or NGFI-A- specific riboprobe in relation to the rat β-actin standard and are expressed as percentage of the Ringer solution group. The ratios of signals for c-fos, junV, and NGFI-A to β-actin in the Ringer solution group were 0.56 ± 0.053, 0.20 ± 0.022, and 1.86 ± 0.34, respectively. 

^*P < 0.05 using one-way ANOVA and Fisher’s partial least-squares difference post-hoc test for significance.