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Effect of mutation at Cys408 on the hydrolysis of p-NP butyrate
| Km (μM) | kcat (s−1) | kcat/Km (s−1 μM−1) | |
|---|---|---|---|
| Est55 | 24.2 ± 2.5 | (7.9 ± 0.3) × 105 | (3.3 ± 0.4) × 104 |
| C408A | 32.8 ± 2.9 | (13.7 ± 0.4) × 105 | (4.2 ± 0.4) × 104 |
| C408G | 23.1 ± 2.7 | (2.2 ± 0.09) × 105 | (0.95 ± 0.17) × 104 |
| C408P | 26.0 ± 3.2 | (6.5 ± 0.3) × 105 | (2.51 ± 0.3) × 104 |
| C408S | 97.5 ± 4.3 | (12.7 ± 0.6) × 105 | (1.1 ± 0.05) × 104 |
| C408V | 28.3 ± 3.8 | (10.7 ± 0.3) × 105 | (4.5 ± 0.6) × 104 |
