TABLE 3.
Gonadal dysgenesis in the offspring of TP; Birm stocks
| Stocks with attached-X ♀♀
|
Stocks with homozygous X ♀♀
|
||||||
|---|---|---|---|---|---|---|---|
| Stock | Replicatea | No. of vials | No. of ♀♀ | % GD ± SEb | No. of vials | No. of ♀♀ | % GD ± SEb |
| Stocks with Birm autosomesc | |||||||
| w | 1a | 25 | 385 | 100.0 ± 0.0 | 23 | 438 | 99.1 ± 0.4 |
| 1b | 23 | 267 | 100.0 ± 0.0 | 30 | 519 | 98.5 ± 0.6 | |
| 2a | 9 | 41 | 100.0 ± 0.0 | 30 | 556 | 97.2 ± 0.9 | |
| 2b | 25 | 293 | 100.0 ± 0.0 | 19 | 360 | 100.0 ± 0.0 | |
| TP5 | 1a | 25 | 367 | 100.0 ± 0.0 | 30 | 584 | 17.1 ± 4.5 |
| 1b | 25 | 355 | 100.0 ± 0.0 | 26 | 473 | 18.1 ± 3.8 | |
| 2a | 25 | 284 | 100.0 ± 0.0 | 27 | 482 | 0.7 ± 0.3 | |
| 2b | 25 | 453 | 100.0 ± 0.0 | 26 | 384 | 1.9 ± 0.8 | |
| TP6 | 1a | 25 | 424 | 100.0 ± 0.0 | 11 | 198 | 0.9 ± 0.9 |
| 2a | 25 | 281 | 99.6 ± 0.4 | 36 | 416 | 1.2 ± 0.8 | |
| 2b | 23 | 261 | 99.6 ± 0.4 | 37 | 507 | 2.3 ± 0.9 | |
| Control attached-X stocksd | |||||||
| w | 1 | 24 | 373 | 100.0 ± 0.0 | |||
| 2 | 24 | 328 | 100.0 ± 0.0 | ||||
| TP5 | 1 | 24 | 460 | 100.0 ± 0.0 | |||
| 2 | 25 | 295 | 99.7 ± 0.2 | ||||
| TP6 | 1 | 24 | 411 | 100.0 ± 0.0 | |||
| 2 | 25 | 398 | 99.5 ± 0.4 | ||||
| π2 | 1 | 22 | 328 | 2.1 ± 1.1 | |||
| Control free-X stocks | |||||||
| w | 30 | 600 | 100.0 ± 0.0 | ||||
| TP5 | 32 | 596 | 85.4 ± 3.4 | ||||
| TP6 | 24 | 262 | 31.6 ± 6.0 | ||||
| M5B#1 | 24 | 277 | 94.8 ± 2.3 | ||||
Among the attached-X stocks, replicates 1, 1a, and 1b carried C(1)DX, y f and replicates 2, 2a, and 2b carried C(1)DX, y w f.
Unweighted mean percentage of GD ± standard error (SE). Note that in the tests of females with attached-X chromosomes, GD is induced by autosomes and a Y chromosome derived from the Harwich P strain whereas, in the tests of females with homozygous X chromosomes, it is induced by autosomes and an X chromosome from this strain.
Stocks with attached-X chromosomes and Birm autosomes were created by repeatedly backcrossing males that carried a particular free X chromosome to attached-X females that carried the Birm autosomes. Homozygous X stocks were created by crossing males from each of the attached-X stocks to M5B#1 females and then using the balancing properties of the M5 chromosome to make the free X chromosome homozygous.
The π2 attached-X stock was derived from a P strain. It contains numerous P elements and carries the double P-element insertion mutation snw on its free X chromosome (Engels 1979b). The only P elements present in the other control attached-X stocks were TP5 or TP6.