Figure 5.—

MMEJ requires multiple polymerases including two translesion bypass polymerases. Survival frequency (A) and the contribution of MMEJ and NHEJ to repair (B) among survivors after induction of HO breaks of strains lacking one or more nonessential DNA polymerases or KU70 were determined by analysis of junctional sequences observed in survivors. (C) Plasmids expressing POL4 or its mutant derivatives were introduced into SLY19-pol4Δ, and the frequency of MMEJ and NHEJ following induction of HO breaks was determined as in A. Each value represents the average from at least three independent experiments ± standard deviation.