Figure 2.—

Constitutive SPS sensor-regulated gene expression in asi1Δ mutants is strictly Dal81 dependent. (A) Strains ptr3Δ asi1Δ (MBY13; dilution series 1) and ptr3Δ asi1Δ dal81−101 (MBY15; dilution series 2–4) carrying plasmids pRS316 (vc), pHK027 (PTR3), or pMB71 (DAL81) were grown in SD supplemented with glutamate, leucine, and histidine (SD). Aliquots of 10-fold serial dilutions were spotted on SD and SD containing AzC (SD + AzC). Plates were incubated at 30°. (B) Wild-type (WT; CAY28) and mutant ssy1Δ (MBY40), asi1Δ (PLY1313), ssy1Δ asi1Δ (CAY206), ssy1Δ asi1Δ dal81Δ (MBY66), asi1Δ dal81Δ (MBY67), and asi1Δ dal81Δ stp1Δ stp2Δ (MBY80) strains were grown and growth characteristics analyzed as in A.