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Dal81 is not required for nuclear localization of Stp1. Indirect immunolocalization of Stp1-HA in DAL81 (CAY60) and dal81Δ (MBY79) cells was performed with anti-HA monoclonal antibodies. Strains carrying plasmid pMB31 (Stp1-HA) were grown in SD and induced 30 min with leucine. Left to right: α-HA monoclonal antibody-dependent Alexa Fluor 488 fluorescence; DAPI staining; and cells viewed by Nomarski optics.
