TABLE 2.
Overview of the phenotype and mapping of the isolated class I mutant lines
| Clones at the distal tip | Clones at the proximal region | Analyzed clones | % at the distal tip | Lethal line | Mapping to | Does not complement to | Identified gene | |
|---|---|---|---|---|---|---|---|---|
| 2L 3267 | 0 | 26 | 26 | 0.0 | Yes | Df(2L)exel6039, 8036 | ||
| 2L 2870 | 0 | 39 | 39 | 0.0 | Yes | |||
| 2L 2677 | 1 | 29 | 30 | 3.3 | Yes | |||
| 2L 2436 | 1 | 15 | 16 | 6.3 | Yes | Df(2L)exel8012, 7021 | ||
| 2L 3186 | 1 | 13 | 14 | 7.1 | Yes | Df(2L)exel7038 | ||
| 2L 3189 | 1 | 12 | 13 | 7.7 | Yes | |||
| 2L 3081 | 4 | 31 | 35 | 11.4 | Yes | Df(2L)exel8038 | ||
| 2L 2881 | 2 | 14 | 16 | 12.5 | Yes | Df(2L)exel6027, 7067 | mhc1 | mhc |
| 2L 3298 | 1 | 6 | 7 | 14.3 | Yes | Df(2L)exel7034 | ||
| 2L 2475 | 1 | 6 | 7 | 14.3 | Yes | Df(2L)exel6042 | 2L1665 | |
| 2L 2653 | 2 | 11 | 13 | 15.4 | Yes | Df(2L)exel6047, 7055 | ||
| 2L 1683 | 2 | 11 | 13 | 15.4 | Yes | Df(2L)exel7038 | ||
| 2L 3297 | 4 | 21 | 25 | 16.0 | Yes | Df(2L)exel8026, 7049 | 2L2896 | stam |
| 2L 2896 | 5 | 24 | 29 | 17.2 | Yes | Df(2L)exel7038, 8026, 7049 | 2L3297 | stam |
| 2L 2828 | 6 | 26 | 32 | 18.8 | Yes | |||
| 2L 2985 | 4 | 15 | 19 | 21.1 | Yes | |||
| 2L 3073 | 3 | 10 | 13 | 23.1 | Yes | |||
| 2L 2845 | 10 | 32 | 42 | 23.8 | Yes | |||
| 2L 2853 | 8 | 25 | 33 | 24.2 | Yes | |||
| 2L 2718 | 2 | 5 | 7 | 28.6 | Yes | |||
| 2L 2775 | 7 | 17 | 24 | 29.2 | Yes | Df(2L)exel6028 | ||
| 2L 1710 | 4 | 9 | 13 | 30.8 | Yes | |||
| 2L 1685 | 7 | 15 | 22 | 31.8 | Yes | Df(2L)exel8003 | ||
| 2L 948 | 9 | 18 | 27 | 33.3 | Yes | |||
| 2L 2921 | 6 | 12 | 18 | 33.3 | Yes | |||
| 2L 1561 | 3 | 6 | 9 | 33.3 | Yes | Df(2L)exel6042 | ||
| 2L 1540 | 12 | 22 | 34 | 35.3 | Yes | Df(2L)exel6044 | ||
| 2L 1809 | 10 | 18 | 28 | 35.7 | Yes | |||
| 2L 2938 | 9 | 16 | 25 | 36.0 | Yes | Df(2L)exel6277 | ||
| 2L 1665 | 9 | 16 | 25 | 36.0 | Yes | Df(2L)exel6042 | 2L2475 | |
| 2L 3179 | 8 | 14 | 22 | 36.4 | Yes | Df(2L)exel7022 | ||
| 2L 2615 | 8 | 14 | 22 | 36.4 | Yes | Df(2L)exel6031 | ||
| 2L 3011 | 13 | 22 | 35 | 37.1 | Yes | Df(2L)exel6042, 8040 | ||
| 2L 1794 | 8 | 13 | 21 | 38.1 | Yes | |||
| 2L 2468 | 1 | 7 | 8 | 12.5 | No | |||
| 2L 2366 | 1 | 7 | 8 | 12.5 | No | |||
| 2L 883 | 4 | 12 | 16 | 25.0 | No | |||
| 2L 3064 | 7 | 15 | 22 | 31.8 | No |
Lines displaying a strict tracheal cell migration phenotype, characterized by the observation of <40% of MARCM clones reaching the distal tip of the air sac primordium (see also Figure 3, B–D), were retained for further analysis (class I mutants). We recovered 38 strains meeting this criterion (see also Table 1). For each line, numbers refer to the amount of MARCM clones observed at the distal tip of the air sac primordium (column 2) and in the proximal region (column 3), to the total number of observed clones (column 4), and to the percentage of MARCM clones localized at the air sac primordium distal tip (column 5; Figure 3). We recovered 34 homozygous lethal lines and 4 homozygous viable lines. Exelixis deficiencies, other independent class I mutants or previously characterized alleles, and names of mutants belonging to the same complementation group as other class I mutants we isolated are indicated in columns 7, 8, and 9, respectively.