TABLE 3.

Overview of the phenotype of the isolated class II mutant lines

	Clones at the distal tip	Clones at the proximal region	Analyzed clones	% at the distal tip	Lethal line
2L 3194	0	7	7	0.0	Yes
2L 1748	0	3	3	0.0	Yes
2L 1682	0	5	5	0.0	Yes
2L 2983	0	1	1	0.0	Yes
2L 2834	0	3	3	0.0	Yes
2L 2798	0	7	7	0.0	Yes
2L 2686	3	23	26	11.5	Yes
2L 3328	3	21	24	12.5	Yes
2L 1738	4	15	19	21.1	No

Class II mutant lines displayed migration phenotypes meeting our screening criteria (<40% of MARCM clones reaching the air sac primordium distal tip). However, class II mutant lines also displayed MARCM clones of reduced size (see also Figure 3, E and F). We recovered nine strains meeting this criterion (see also Table 1). For each line, numbers refer to the amount of MARCM clones observed at the distal tip of the air sac primordium (column 2) and in the proximal region (column 3), to the total number of observed clones (column 4), and to the percentage of MARCM clones localized at the distal tip of the air sac primordium (column 5; Figure 3, E and F). We recovered eight homozygous lethal lines and one homozygous viable line.