TABLE 2.
The C2-like domain is not absolutely required for Pkn function in vivo
| Transgenic line | % rescue (n) | Relative mRNA expression |
|---|---|---|
| PknP/CyO | 0 (729) | − |
| Pkn(11)/CyO (37) | 74.5 (1514) | ++ |
| ΔC2/TM2 (3A) | 38.7 (475) | ++ |
| ΔC2/TM2 (3B) | 9.7 (582) | ++ |
| ΔC2/TM2 (3C) | 4.0 (506) | + |
| ΔC2/TM6B (1W) | 7.4 (530) | +++ |
Percentage of rescue was determined by counting the number of CyO-balanced and nonbalanced flies and calculating the percentage of flies that could be rescued to adulthood on the basis of expected Mendelian ratios. The total number (n) of flies counted is shown in parentheses. Relative mRNA expression of the transgenes was estimated from ethidium bromide-agarose gels of RT–PCR products. For each transgene: +++, the line that gave the strongest PCR band; ++, the line with the second stongest bands; +, the weakest; −, no expression.