TABLE 3.
The kinase domain of PKC53E can functionally substitute for that of Pkn
| Transgenic line | % rescue (n) | Relative mRNA expression |
|---|---|---|
| PknP/CyO | 0 (896) | − |
| Pkn(11)/CyO (37) | 74.1 (1069) | ND |
| Pkn-PKC53E/TM6B (K1AA) | 20.4 (464) | ++ |
| Pkn-PKC53E/TM2 (L4-AD) | 1.2 (681) | +++ |
| Pkn-PKC53E/TM2 (M1) | 20.0 (451) | ++ |
| Pkn-PKC53E/CyO (3B-M2) | 13.6 (725) | ++ |
| Pkn-PKC53E/CyO (32-L2) | 23.2 (914) | ++ |
Percentage of rescue was determined by counting the number of CyO-balanced and nonbalanced flies and calculating the percentage of flies that could be rescued to adulthood on the basis of expected Mendelian ratios. The total number (n) of flies counted is shown in parentheses. Relative mRNA expression of the transgenes was estimated from ethidium bromide-agarose gels of RT–PCR products. For each transgene: +++, the line that gave the strongest PCR band; ++, the line with the second stongest bands; +, the weakest; −, no expression. ND, not determined.