Figure 1.—

Identification of the ego-2 gene. (A) ego-2 was localized to a 137-kb region by mapping relative to SNPs. Relative positions of SNPs are shown; the cosmid or yeast artificial chromosome names and chromosomal coordinates of the SNPs are listed. lin-11 non-ego-2(om33) recombinants were used to determine the left boundary of the ego-2 region, and unc-75 non-ego-2 recombinants were used to determine the right boundary of the ego-2 region. The number of recombinants recovered within each interval is indicated. (B) Relative locations of the 21 predicted genes in the ego-2 region. (C) Genefinder and ORFeome gene models for Y53H1C.2 are compared with the cDNA sequence that we obtained. Exons are numbered. The corresponding partial cDNA, yk1162g08.2, is indicated. Yk1162g08.2 was previously sequenced at the ends (boxes), but not within the central region (dashed line). We generated dsRNA feeding clones with yk1162g08.2 and genomic DNA corresponding to fragments “a” and “b” as inserts in the plasmid vector (see materials and methods).