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. 2007 Aug;176(4):2265–2277. doi: 10.1534/genetics.107.071225

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Copyright © 2007 by the Genetics Society of America

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Figure 2.— — Enhancement of the glp-1(ts) germline phenotype by loss of ego-2 function. All animals were raised at 20°. An asterisk indicates the distal end of the gonad arm. MZ, mitotic zone; TZ, transition zone (leptotene–zygotene stage); PZ, pachytene zone; sp, sperm; arrows, oocyte; arrowheads in D, sperm. (A) Development of the glp-1(bn18ts) adult hermaphrodite germline is normal. Mitotic and meiotic germ cells, developing oocytes, and sperm are present. (B) Mitosis is maintained in ego-2(om33) unc-75 and (C) ego-2(RNAi) germlines. (D) Glp-1 sterile phenotype caused by ego-2 (Y53H1C.2) RNAi in a glp-1(bn18ts) background. In this animal, mitotic proliferation ceased early in larval development and germ cells differentiated as sperm. This phenotype resembles that of ego-2(om33);glp-1(bn18ts) sterile hermaphrodites (see Qiao et al. 1995).