TABLE 1.
The loss of ego-2 function enhances glp-1(bn18ts) in the germline
| Genotype | Adults/brood (N) | % Glp-1 sterile (n) |
|---|---|---|
| glp-1(bn18ts) | 191 ± 11 (5) | 1.4 (957) |
| unc-75 | 190 ± 10 (6) | 0 (1138) |
| ego-2(om33) unc-75 | 192 ± 11 (6) | 0 (1149) |
| unc-75; glp-1(bn18) | 135 ± 3 (12) | 0.1 (1620) |
| ego-2(om33) unc75; glp-1(bn18) | 90 ± 12 (10) | 8.5 (896) |
| ego-2(om33/tm2272) | 28 ± 3 (14) | 0 (398) |
| ego-2(om33/tm2272); glp-1(bn18)a | ND | 88 (175) |
| ego-2(RNAi)b | 190 ± 8 (12) | 0 (2277) |
| ego-2(RNAi);unc-32 glp-1(bn18ts)b,c | 134 ± 7 (31) | 45 (4160) |
Experiments were done at 20°. N, number of broods scored; n, number of animals scored; ND, not determined. “Glp-1 sterile” is the percentage of germlines in which all germ cells had prematurely exited mitosis, entered meiosis, and differentiated. Number following the “±” is the standard error of the mean.
Animals were obtained as progeny of ego-2(om33) unc-75/ego-2(tm2272); glp-1(bn18/+) mothers (whose average brood size was 36 ± 4.5). A total of 321 progeny (nine broods) were scored; 47/214 ego-2(om33) unc-75/ego-2(tm2272) animals were Glp-1 sterile, which is 88% (47/53) of the expected number of glp-1(bn18/bn18) animals. In addition, 8/107 ego-2(om33) unc-75 animals were Glp-1 sterile, which is 30% (8/27) of the expected number of glp-1(bn18/bn18) animals.
Data are the average of four independent feeding experiments.
A similar degree of enhancement was observed in the absence of the unc-32 marker (data not shown).