TABLE 6.
alx-1(0) suppresses the enhancement of glp-1(ts) by ego-2 in the germline
| Genotype | Brood size (N) | % Glp-1 sterile (n) |
|---|---|---|
| ego-2(RNAi) | 192 ± 6 (3) | 0 (575) |
| alx-1(0) | 234 ± 6 (19) | 0.1 (4453) |
| unc-36 glp-1(bn18) | 171 ± 9 (11) | 1.0 (1877) |
| unc-36 glp-1(bn18) alx-1(0) | 178 ± 7 (10) | 0.1 (1783) |
| ego-2(RNAi); alx-1(0) | 186 ± 3 (6) | 0 (1114) |
| ego-2(RNAi); unc-36 glp-1(bn18)a | 168 ± 7 (11) | 27 (1856) |
| ego-2(RNAi); unc-36 glp-1(bn18) alx-1(0)a | 177 ± 8 (8) | 11 (1413) |
All tests were done at 20°. “Glp-1 sterile” is as described in the Table 1 legend. N, number of broods scored; n, number of animals scored.
a
Two independent RNAi experiments were done. We consistently observed reduced enhancement of glp-1 in alx-1(0) vs. alx-1(+) animals.