Figure 2.—

AlRPW8.2 confers powdery mildew resistance in Col-0. (A) A 2.3-kb genomic fragment containing the AlRPW8.2 gene and its native promoter was introduced to Col-gl background and the transgenic plants were inoculated with E. cichoracearum USCS1. Disease phenotypes were scored and typical infected leaves were photographed at 10 days post-inoculation (dpi). (B) The AlRPW8.2 gene was expressed under control of the 35S promoter in Col-gl background. Plants of ∼10% (3 of 29) transgenic lines showed SHL and 4-week-old T3 plants of one line with the most severe SHL were shown together with the wild type. Arrows indicate dead or dying leaves. Bar, 1 cm.