TABLE 1.
Primers used for creating constructs
| Primer name | Sequencea |
|---|---|
| SME-STARTdel | 5′-CAATGACAGTTAATAGGTAAAGTTACTAGTAACAAAGTAAATTTTAAAACGGCTAGC-3′ |
| SME-Sac | 5′-GGGGCGGAGCTCAACTCATTCAACACTCGG-3′ |
| SME-BamHI | 5′-GGGGCGGGATCCGCGTCAAGGCCACAGTCAGCTCTAACGC-3′ |
| SME-C69V-Top | 5′-GCGGTTCCCTTTAGTCTCAAGCTTTAAAGGTTTTTTGGCGGCTGC-3′ |
| SME-C238V-Top | 5′-GGTGACAAAACTGGGAGCGTTGGCGCCTATGGTACTGCGAATG-3′ |
| SME-E166Q-Top | 5′-GGTTAGATCGCTGGCAGCTGGAACTTAACACTGC-3′ |
| SME-E166Q-Bot | 5′-GCAGTGTTAAGTTCCAGCTGCCAGCGATCTAACC-3′ |
| SME-S70A-Top | 5′-GCGGTTCCCTTTATGCGCTTCATTTAAAGGTTTTTTGGCGGCTGC-3′ |
| SME-S70A-Bot | 5′-GCAGCCGCCAAAAAACCTTTAAATGAAGCGCATAAAGGGAACCGC-3′ |
| PDbla1 | 5′-CGGGGAGCTCGTTTCTTAGACGTCAGGTGG-3′ |
| PDbla2 | 5′-CCCCGTCTAGACTTACCAATGCTTAATCAGT-3′ |
| TEM-AgeI-Top | 5′-CATTTTGCCTACCGGTTTTTGCTCAC-3′ |
| TEM-AgeI-Bot | 5′-GTGAGCAAAAACCGGTAGGCAAAATC-3′ |
| TEM-SME | 5′-GCCTACCGGTTTTTGCTAACAAAAGTGATGCTGC-3′ |
| Pbla-ssSME-Top | 5′-CAATAATATTGAAAAAGGAAGAGTATGTCAAACAAAGTAAATTTTAAAACGGCTAGC-3′ |
| Pbla-ssSME-Bot | 5′-GCTAGCCGTTTTAAAATTTACTTTGTTTGACATACTCTTCCTTTTTCAATATTATTG-3′ |
| ssSME-mTEM-Top | 5′-GCTTTGTCGGCATTTAATGCTCATGCTCACCCAGAAACGCTGG-3′ |
| ssSME-mTEM-Bot | 5′-CCAGCGTTTCTGGGTGAGCATGAGCATTAAATGCCGACAAAGC-3′ |
| pyrF-bla-top | 5′-CACGCGATTGTCGTCTGAAGGTCGGCAAAGAGATGGTTTCTTAGACGTCAGGTGGCACTTTT CGGGGAAATG-3′ |
| pyrF-bla-bot | 5′-GCGGTGCATCTTTGCCAAACGGAACCAGTGCCTCTTACCAATGCTTAATCAGTGAGGCACCTA TCTCAGCG-3′ |
| SME-1STOP-pyrF384bot | 5′-GGCTTCCATGCTGGTCAACACTGTCAATTAATCAATTGCCTGAATTGC-3′ |
a
Engineered restriction sites utilized for cloning and/or screening are underlined.