Figure 3.—
Pi36 gene complementation test and molecular analysis of the transgenic lines. (A) Resistance phenotypes of the Pi36 gene donor cv. Kasalath and its receptor cv. Q1063 as well as transgenic plants of two T2 lines segregated into 3R:1S against isolate CHL39. R, resistant; S, susceptible. (B) Cosegregation analysis of the resistance phenotype with the transgenes. The amplified fragment with the primer pair CRG4F and CRG4R was subsequently digested with HaeIII, and the digested product was subjected to 1.5% agarose gel electrophoresis. M represents standard molecular weight marker DL2000.