Skip to main content
. Author manuscript; available in PMC: 2007 Aug 22.
Published in final edited form as: Neurochem Int. 2007 Feb 23;50(6):834–847. doi: 10.1016/j.neuint.2007.02.003

Table 3.

Quantitative RT-PCR analysis of select candidate genes

Genes Band size (bp) DNA Gel Con - H2O2 H2O2/con mRNA fold difference Z-Ratio H2O2/Con
CCND1
cyclin D1		 720 graphic file with name nihms24168t1.jpg + 4.1 + 11.7
STAT5B
Signal transducer and activator of transcription 5B		 326 graphic file with name nihms24168t2.jpg + 3.0 + 4.0
Jun B
proto-oncogene		 630 graphic file with name nihms24168t3.jpg + 2.8 + 4.0
PAWR
apoptosis, WT1, regulator		 852 graphic file with name nihms24168t4.jpg + 2.9 + 3.7
PSMD3
26S proteosome subunit, non- ATPase, 3		 976 graphic file with name nihms24168t5.jpg + 2.8 + 3.0
MAP2K
mitogen-activated protein kinase kinase 1		 1703 graphic file with name nihms24168t6.jpg − 1.1 − 3.2
GAPDH
Glycerol Aldehyde Dehydrogenase		 640 graphic file with name nihms24168t7.jpg + 1.0 -

Total RNA was extracted from control and H2O2-treated N27 cells using the Trizol reagent. RT-PCR was performed using the primers designed against the rat species (see methods section for more details).