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. Author manuscript; available in PMC: 2007 Aug 22.
Published in final edited form as: Neurochem Int. 2007 Feb 23;50(6):834–847. doi: 10.1016/j.neuint.2007.02.003

Table 4.

Western blot analysis of select candidate genes

Genes Band size (kDa) Western blot Con - H2O2 Protein H2O2/Con fold difference Z-Ratio H2O2/Con
Jun B
proto-oncogene		 39 graphic file with name nihms24168t8.jpg + 3.0 + 4.0
STAT5B
Signal transducer and activator of transcription 5B		 80 graphic file with name nihms24168t9.jpg + 2.5 + 4.0
MEKK4
mitogen-activated protein kinase kinase kinase kinase 2		 45 graphic file with name nihms24168t10.jpg + 3.6 + 3.8
PP2B
protein phosphatase 2B (calcineurin A gamma)		 60 graphic file with name nihms24168t11.jpg + 2.3 + 3.0
BNIP3
BCL2/adenovirus E1B interacting protein 3		 19 graphic file with name nihms24168t12.jpg − 3.0 − 3.8
β–actin 43 graphic file with name nihms24168t13.jpg + 1.0 -

Control and H2O2-treated cell extracts were separated on 10–15% SDS-PAGE followed by western blot. Primary antibodies that would recognize the rat species were used in this study (see methods section for more details).