Table 4.
Genes | Band size (kDa) | Western blot Con - H2O2 | Protein H2O2/Con fold difference | Z-Ratio H2O2/Con |
---|---|---|---|---|
Jun B
proto-oncogene |
39 | + 3.0 | + 4.0 | |
STAT5B
Signal transducer and activator of transcription 5B |
80 | + 2.5 | + 4.0 | |
MEKK4
mitogen-activated protein kinase kinase kinase kinase 2 |
45 | + 3.6 | + 3.8 | |
PP2B
protein phosphatase 2B (calcineurin A gamma) |
60 | + 2.3 | + 3.0 | |
BNIP3
BCL2/adenovirus E1B interacting protein 3 |
19 | − 3.0 | − 3.8 | |
β–actin | 43 | + 1.0 | - |
Control and H2O2-treated cell extracts were separated on 10–15% SDS-PAGE followed by western blot. Primary antibodies that would recognize the rat species were used in this study (see methods section for more details).